Essa Dirandeh; Mohammad Kazemi Fard; Tannaz Saberifar
Volume 24, Issue 3 , October 2022, , Pages 373-382
Abstract
To investigate the effect of fatty liver on insulin resistance in the liver of laying hens, an experiment using 80 laying hens of commercial line strains (w-36) after peak production (age 43 weeks) for eight weeks were performedin a completely randomized design with two treatments. The experimental treatments ...
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To investigate the effect of fatty liver on insulin resistance in the liver of laying hens, an experiment using 80 laying hens of commercial line strains (w-36) after peak production (age 43 weeks) for eight weeks were performedin a completely randomized design with two treatments. The experimental treatments included control group (no injection) and the estradiol group (injection of two mg estradiol benzoate per kg body weight). In order to induce fatty liver disease, the injection of 17-beta estradiol started from the third week of experiment (age 46), and was performed three times a week for 21 days. Blood samples were taken to evaluate the concentration of triglycerides, cholesterol and liver enzymes (aspartate transaminase (AST), alanin transaminase (ALT) and alkaline phosphatase (ALP) at the end of the experiment using 20 hens from each treatment. At the end of experiment, five hens of each treatment were selected and sacrificed, then 50 g of liver tissue was removed to study gene expression of insulin receptor (InR), glucose transporter1 (Glut1), sterol regulatory element binding protein (SREBP1), Ribosomal S6 kinase1 (S6K1), Target of Rapamycin (TOR) and Forkhead box protein O1 (FOXO1). The results showed that the injection of estradiol induced fatty liver and increased plasma concentrations of cholesterol and triglyceride as well as activity of AST, ALT and ALP. In hens with fatty liver, expression of FOXO1 (4.1-fold), TOR (3.9-fold), S6K1 (3.3-fold) genes increased, and conversely, expression of InR (4.6-fold), Glut1 (7.5-fold) decreased. In conclusion results of the present study showed that the fatty liver induction in laying hens increased expression of insulin resistance-related genes.
Essa Dirandeh; Mohammad Kazemi Fard; Tannaz Saberifar
Volume 24, Issue 2 , July 2022, , Pages 227-235
Abstract
The occurrence and spread of inflammation can affect the quantity and quality of eggs in laying hens. Therefore, the aim of this study was to evaluate the effect of soy Gensitein (GEN)on systemic inflammatory status and performances of laying hens post-peak. The research was performed in a completely ...
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The occurrence and spread of inflammation can affect the quantity and quality of eggs in laying hens. Therefore, the aim of this study was to evaluate the effect of soy Gensitein (GEN)on systemic inflammatory status and performances of laying hens post-peak. The research was performed in a completely randomized design, during a period of about 8 weeks, using 80 laying hens of High Line W-36 strains (older than 43 weeks). Experimental treatments were control group (basal diet) and genistein group (basal diet+ 20mg GEN/kg of diet). At the end of experiment, five hens from each treatment were laughter and 50 g of liver sample were taken for gene expression of IL-1, IL-2, TNF-α, COX-1 and COX-2. Results showed that soy GEN decreased gene expression of IL-1 (245 fold), IL-2 (3.53 fold), IL-6 (2.68 fold), TNF-α (4.83 fold), COX-1 (3.92 fold) and COX-2 (1.73 fold) compared to the control group. Laying hens fed GEN diets had higher feed intake and better conversion ratio and had higher egg production rates than the control group.The results of this study showed that soy GEN could reduce inflammation in the liver of laying hens post-peak and improve production performances.